The synthesis of cozymase from nicotinic acid and its derivatives by Lactobacillus arabinosus 17-5.
نویسندگان
چکیده
to leave a phenol-insoluble mucoid. This contains a substance with the characteristics of a mucopolysaccharide-protein complex. 5. The water-soluble mucoids contain a complex which is formed by interaction between the phenolinsoluble mucoids and a water-soluble protein of the cornea. 6. The acid mucopolysaccharide can be prepared free from protein by incubation with trypsin. Galactose, glucosamine and galactosamine have been identified as constituents of the mucopolysaccharide, by chromatography and ionophoresis on ifiter paper. 7. The corneal mucopolysaccharide is not a substrate for hyaluronidase. 8. Gel-forming proteins have been isolated from the insoluble mucoid and found to contain the same amino-acids as gelatin, but to differ from it in their electrophoretic behaviour. 9. It is suggested that in situ the acid mucopolysaccharide is combined with proteins of the collagen group.
منابع مشابه
The behaviour of Lactobacillus arabinosus towards nicotinic acid and its derivatives.
These investigations originated in studying the basis of a method for microbiological assay of nicotinic acid (McIlwain & Stanley, 1948). The method was that in which acid produced from glucose by Lactobacillus arabinosus 17-5 is titrated with alkali after a period of incubation of about 3 days. The assay depends on the empirical observation that the quantity of titratable acid produced during ...
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Lactobacillus arabinosus 17-5 has been widely used as an assay organism for nicotinic acid (NA) since the development of the method by Snell and Wright (1). Although it has been realized that other substances present in tissue extracts may interfere with the bioassay, the nature of such substances has not been elucidated. During an investigation of tryptophan metabolism in the pea plant, we stu...
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عنوان ژورنال:
- The Biochemical journal
دوره 51 3 شماره
صفحات -
تاریخ انتشار 1952